Dan Brune Research Scientist, Department of Chemistry and Biochemistry

Research Interests:

My research interests include improvement of techniques in protein analysis and dissimilatory sulfur metabolism in photosynthetic bacteria.

I am in charge of a protein analysis and synthesis laboratory. Equipment in the laboratory includes a Porton (Beckman) 2090 protein sequencer, a Hewlett-Packard AminoQuant amino acid analyzer, a Vestec laser desorption time-of-țight mass spectrometer, a Millipore 9050 peptide synthesizer, and Beckman high pressure liquid chromatography and capillary electrophoresis equipment. I also oversee equipment in a spectroscopy laboratory that contains a JASCO 710 spectropolarimeter, a Hitachi UV-Vis spectrophotometer, and a Shimadzu țuorometer. In addition to analyzing samples for other researchers using the protein sequencer, amino acid analyzer, and mass spectrometer and synthesizing polypeptides using the peptide synthesizer, I supervise and train students in the use of any of the equipment that is useful to their research. These services are provided to researchers at ASU for fees that cover only the costs of routine maintenance and replacement of consumables.

Selected Publications:

Lee, W.-Y., Brune, D.C., LoBrutto, R. and Blankenship, R.E. (1995) Isolation, characterization, and primary structure of rubredoxin from the photosynthetic bacterium, Heliobacillus mobilis. Arch. Biochem. Biophys. 318: 80­88.

Brune, D.C. (1995) Sulfur compounds as photosynthetic electron donors. In: Anoxygenic Photosynthetic Bacteria, Blankenship, R.E., Madigan, M.T., Bauer, C.E. (eds), pp 847­870. Kluwer Academic Publishers, Dordrecht.

Brune, D.C. (1995) Isolation and characterization of sulfur globule proteins from Chromatium vinosum and Thiocapsa roseopersicina. Arch. Microbiol. 163: 391­399.

Brune, D.C., Hartzfeld, D.G. and Johnson, T.W. (1995) Cysteine modiȚcation with acrylonitrile for amino acid analysis and protein sequencing. In: Techniques in Protein Chemistry VII, Marshak, D.R. (ed), in press.

Pettit, G.R., Xu, J., Chichacz, Z.A., Wiliams, M.D., Dorsaz, A.-C., Brune, D.C., Boyd, M.R. and Cerny, R.L. (1994) Antineoplastic agents 315. Isolation and structure of the marine sponge cancer cell growth inhibitor Phakellistatin 5. Bioorganic Med. Chem. Lett. 4: 2091­2096.

Contact Information:

Tel: 1-(480) 965-0795
Fax: 1-(480) 965-2747
Email: Brune@asuchm.la.asu.edu

Office Room Number: Goldwater GW 682
Lab Room Number: Goldwater GW 682

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