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PART I, Molecular Biology Background
August 21 |
Introduction and review; chapters 1, 2 and start of 3 |
August 23 |
Review: DNA, RNA, and proteins; chapter 3 |
August 28 |
Restriction mapping, cloning, Southern blotting; chapter 4 |
August 30 |
cDNA, DNA libraries; prokaryotic transformation; chapter 4 |
September 4 |
DNA synthesis and sequencing; chapter 5 |
September 6 |
DNA amplification (PCR); chapter 5 |
September 11 |
Quiz I (chapters 1-5) |
PART II, Applications and Implications of DNA Technology: the Basics
September 13 |
Manipulation of gene expression in prokaryotes; plasmids and genomic integration; chapter 6 |
September 18 |
Protein overexpression systems: chapters 6 and 7 |
September 20 |
Directed and combinatorial mutagenesis: examples; chapter 8 |
September 25 |
Mutagenesis procedures: PCR, oligonucleotide-directed, combinatorial, random, transposon mutagenesis, protein engineering; chapter 8 |
September 27 |
DNA diagnostics vs. ELISA; chapter 9 |
October 2 |
Producing human proteins in bacteria; expression optimization; chapter 10 |
October 4 |
Vaccines: subunit and peptide vaccines; chapter 11 |
October 9 |
Quiz II (chapters 6-11) |
PART III, Applications and Implications of DNA Technology: Industrial and Field Applications
| October 11 | Bioremediation and biomass utilization (Bruce Rittmann); chapter 13 |
October 16 |
Plant genetic engineering; chapter 17 |
October 18 |
Transgenic plants; chapter 18 |
October 23 |
Examples of pathway engineering and overexpression to produce useful organic compounds; start on bioremediation; chapter 12 and 13 |
October 25 |
Microbes aiding in crop productivity and biological insecticides: the Bt toxin; chapters 14 and 15 |
October 30 |
Large-scale production using microorganisms: examples and scale-up challenges; chapter 16 |
November 1 |
Transgenic animals; chapter 19 |
November 6 |
Quiz III (chapters 12-19) |
PART IV, Genomics and Impacts on Biomedicine, Bioethics, and the Future of Biotechnology
November 8 |
Concept of linkage mapping to identify human disease genes; chapter 20 |
November 13 |
Genome projects: concepts, scope, and implications |
November 15 |
A primer to genomics and proteomics |
November 20 |
Bioterrorism; regulation and patenting rules; chapters 21-22 |
November 27 |
Stem cell research: facts and fiction |
November 29 |
Perspective on future directions in biotechnology |
December 4 |
Quiz IV |
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Center for Bioenergy & Photosynthesis Arizona State University Box 871604 Room PSD 209 Tempe, AZ 85287-1604
18 November 2007 |
phone: (480) 965-1963 fax: (480) 965-2747 |