Arizona State University College of Liberal Arts and Sciences

Bio343

Detailed Lecture Schedule

(subject to change)


PART I, Molecular Biology Background

August 26

Introduction and review; chapters 1, 2 and start of 3

August 28

Review: DNA, RNA, and proteins; chapter 3

September 2

Restriction mapping, cloning, Southern blotting; chapter 4

September 4

cDNA, DNA libraries; prokaryotic transformation; chapter 4

September 9

DNA synthesis and sequencing; chapter 5

September 11

DNA amplification (PCR); chapter 5

September 16

Quiz I (chapters 1-5)

PART II, Applications and Implications of DNA Technology: the Basics

September 18

Manipulation of gene expression in prokaryotes; plasmids and genomic integration; chapter 6

September 23

Protein overexpression systems: chapters 6 and 7

September 25

Directed and combinatorial mutagenesis: examples; chapter 8

September 30

Mutagenesis procedures: PCR, oligonucleotide-directed, combinatorial, random, transposon mutagenesis, protein engineering; chapter 8

October 2

DNA diagnostics vs. ELISA; chapter 9

October 7

Producing human proteins in bacteria; expression optimization; chapter 10

October 9

Vaccines: subunit and peptide vaccines; chapter 11

October 14

Quiz II (chapters 6-11)

PART III, Genomics and Impacts on Biomedicine, Bioethics, and the Future of Biotechnology

  

October 16

Concept of linkage mapping to identify human disease genes; chapter 20

October 21

Genome projects: concepts, scope, and implications

October 23

A primer to genomics and proteomics

October 28

Bioterrorism; regulation and patenting rules; chapters 21-22

October 30

Stem cell research: facts and fiction

November 4

Perspective on future directions in biotechnology

November 6

Quiz III (Chapters 20-22)

 

 

PART IV, Applications and Implications of DNA Technology: Industrial and Field Applications

November 13

Examples of pathway engineering and overexpression to produce useful organic compounds; start on bioremediation; chapter 12 and 13

November 18

Bioremediation and biomass utilization; chapter 13

November 20

Microbes aiding in crop productivity and biological insecticides: the Bt toxin; chapters 14 and 15

November 25

Large-scale production using microorganisms: examples and scale-up challenges; chapter 16

December 2

Plant genetic engineering; Transgenic plants; chapters 17-18

December 4

Transgenic animals; chapter 19

December 9

Quiz IV
   

Return to Contents

Instructors | Aims
Lecture Part: Schedule | Expected Background & Textbook Info | Historical Perspective
Intro to Biotechnology | DNA, RNA and Protein Synthesis | Chemical Synthesis, Sequencing, and Amplification of DNA |
Directed Mutagenesis and Protein Engineering | Vaccines | Antibiotics & Proteins | Bioremediation |
Microbial Insecticides | Plant Genetic Engineering: Methodology | Plant Genetic Engineering: Applications | Transgenic Animals
Human Molecular Genetics | Regulatory & Ethical Aspects | Biotech Inventions | Additional Materials
Lab Part: Aims and Expectations | Schedule

Center for Bioenergy & Photosynthesis

Arizona State University

Box 871604

Room PSD 209

Tempe, AZ 85287-1604

 

22 August 2008

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fax: (480) 965-2747

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